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Analysis of cell-free nucleic acids and its potential clinical application.
Pazourková, Eva ; Korabečná, Marie (advisor) ; Drábek, Jiří (referee) ; Vodička, Radek (referee)
This work presents the results ofour research of cell-free nucleic acids (cfNA). The first part shows changes in methylation patterns of immune response genes promoters that are detectable in plasma during the hemodialysis sessions and also differences in methylation between patients and healthy subjects. Alterations include genes that play their role in the regulation of hematopoiesis and these changes are in close relation with the need of anemia therapy. In the other plasma cfNA study we detected miRNA signatures in patients with acute myeloid leukemia at diagnosis (6 highly abundant miRNAs found) and in remission achieved after standard chemotherapy (trend to n01malization, lower levels ofthese miRNAs). Another part of work presents data from the study of potential non-invasive biomarker of bladder cancer. The amounts of cfDNA in urine are higher in patients than in healthy subjects and there were found 5 down-regulated miRNAs. Simultaneously it was established set of 30 miRNAs that are constantly present in urine supematants independently on sex, age and healthy status of subjects. The last part presents analysis ofcell-free fetal DNA. We analyzed differences between a new quantification method - droplet digital PCR and real-time PCR which is used routinely nowadays. Slightly more precise was...
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Acquired hypercoagulable states focus on lupus anticoagulans
ČECHOVÁ, Magda
Hypercoagulable states (thrombophilia) can be defined as a group of inherited or aquired conditions that are associated with predisposition to venous thrombosis, arterial thrombosis or both. Inherited, but also some aquired conditions can be demonstrated by virtue of special laboratories methods. One of those methods is investigation of lupus anticoagulans, which is antibody against phospholipids. These antibodies can cause enhanced blood coagulation, which could later lead to the thrombosis. An appropriate procedure how to investigate lupus anticoagulans was defined.
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